Lab Chemical
PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid
Product code RXSOL-60-6605-260
PIPES BUFFER piperazine NN-Bis-2-Ethene Sulfonic acid
Description
PIPES Free Acid (Piperazine-N,N'-bis(2-ethane sulfonic acid)) PIPES is a biological buffer (zwitterionic) designed by Good, et al. and typically referred to as Good's buffer useful in cell culture media formulations.
Application
Application
PIPES has two pKa values. One pKa (6.76 at 25°C) is near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. The second pKa value is at 2.67 with a buffer range of from 1.5 - 3.5. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.